《植物生理学报》 2015, 51(2): 197-206

小立碗藓MADS-box基因家族的系统进化分析

易吉明¹, 黄婷¹, 黄勇^1,2, 陈东红^1,2,*

湖南农业大学¹生物科学技术学院, ²湖南省作物种植资源创新和利用重点实验室, 长沙410128

通信作者：陈东红；E-mail: chendh212@163.com；Tel: 0731-84673684

摘　要：

苔藓植物作为最早出现的陆生植物的代表, 生活周期仍以配子体为主, 在植物进化史上占据着重要的地位。另外, 同源异型基因MADS-box家族广泛参与植物的生长发育和形态构建。因此, 对苔藓植物MADS-box家族的分析有助于了解苔藓植物出现过程中发生的重要分子事件和关键器官革新。我们基于最新公布的小立碗藓基因组数据库确定了20个带有典型MADS结构域的MADS-box基因, 其中11个MIKC^*型、5个MIKC^C型、4个I型, 并对它们进行了染色体定位、外显子-内含子基因结构、蛋白结构域组成、系统进化构建等分析, 为进一步阐明小立碗藓MADS-box基因的功能提供了准确的信息资源。

关键词：小立碗藓; MADS-box; MIKC; 结构域组成; 系统进化分析

收稿：2014-11-26   修定：2015-02-05

资助：湖南省教育厅青年项目(13B045)和湖南省科技厅青年基金项目(2015JJ3075)。

Phylogenetic Analysis of MADS-box Gene Family in Physcomitrella patens

YI Ji-Ming¹, HUANG Ting¹, HUANG Yong^1,2, CHEN Dong-Hong^1,2,*

¹School of Bioscience and Biotechnology, ²Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China

Corresponding author: CHEN Dong-Hong; E-mail: chendh212@163.com; Tel: 0731-84673684

Abstract:

On behalf of the earliest land plant with the predominant gametophyte generation in the life cycle, bryophyte takes up the important position during plant evolution. Additionally, MADS-box homoetic gene family is widely involved in plant growth and development or morphological architecture. Therefore, it is informative to understand the important molecular events and key organ innovations during the emergence of bryophyte by analyzing moss MADS-box family. Here, we identified 20 MADS-box genes with typical MADS domain in Physcomitrella patens, including 11 members of MIKC^*-type, 5 of MIKC^C-type, and 4 of type-I based on the latest genomic database. Then we investigated the chromosome location, exon-intron gene structure, domain architecture, and phylogenetic relationship on moss MADS-box members. These results provide an accurate information platform for further unveiling the function of moss MADS-box genes.

Key words: Physcomitrella patens; MADS-box; MIKC; domain architecture; phylogenetic analysis